Proliferating cell nuclear antigen (PCNA) immunolabeling as a prognostic factor in invasive ductal carcinoma of the breast in Taiwan. Chu JS, Huang CS, Chang KJ. Cancer Lett. 1998 Sep 25;131 (2):145-52. PMID 985124 . Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities
The gene view histogram is a graphical view of mutations across PCNA. These mutations are displayed at the amino acid level across the full length of the gene by default. Restrict the view to a region of the gene by dragging across the histogram to highlight the region of interest, or by using the sliders in the filters panel to the left This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand General description of the gene and the encoded protein (s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project. Gene namei. Official gene symbol, which is typically a short form of the gene name, according to HGNC. PCNA. Gene descriptioni Functional Associations PCNA has 13,524 functional associations with biological entities spanning 8 categories (molecular profile, organism, functional term, phrase or reference, disease, phenotype or trait, chemical, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 94 datasets
PCNA GEO Profiles, NCBI Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. Latest Publications: PCNA (cancer-related) Chai L, Yang G MiR-216a-5p targets TCTN1 to inhibit cell proliferation and induce apoptosis in esophageal squamous cell carcinoma Duplicated PCNA genes seem to have slightly different functions with one gene encoding the main PCNA protein showing all, or nearly all, the features characteristic of PCNA while a second gene is most likely involved in some aspects of the cell response to DNA-damaging factors by Gene › by Protein › PCNA Antibodies; PCNA Antibodies . PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT)
. Anolis carolinensis (green anole) 1 gene. ENSACAG00000000385 PCNA; Compare expression [+] Amniota: 16 species. Homo sapiens; Mus musculus; Pan troglodytes; Gorilla gorilla; Macaca mulatta; Rattus norvegicus; Bos taurus; Sus scrofa; Equus caballu Gene information about ENSG00000132646 / PCNA - proliferating cell nuclear antige We have previously defined the promoter elements, sites IIa and IIb, in the rice proliferating cell nuclear antigen (PCNA) gene that are essential for meristematic tissue-specific expression. In this study, we isolated and characterized cDNAs encoding proteins that specifically bind to sites IIa and IIb
PCNA gene product. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage,. Rank scores of expression calls are normalized across genes, conditions and species. Low score means that the gene is highly expressed in the condition. Max rank score in all spe
PCNA TTD Gene Abbreviation T21782 TTD Target ID Gene Info: Publications: DGIdb (v4.2.0 - sha1 afd9f30b) • Last updated 2020-10-21. PCLAF (PCNA Clamp Associated Factor) is a Protein Coding gene. Diseases associated with PCLAF include Neuroaspergillosis and Hepatitis C Virus.Among its related pathways are DNA Double-Strand Break Repair and Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template.An important paralog of this gene is ENSG00000259316
Gene target information for PCNA - proliferating cell nuclear antigen (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments . Fig. 2b shows a blot hybridized with the ZmPCNA1 3′ non-translated region probe, where one band can be seen in each of the lanes.This membrane was stripped and reprobed with the ZmPCNA2 3′ non-translated region probe (Fig. 2c), where single different bands. PCNA Gene : Protein Name: Proliferating cell nuclear antigen Uniprot : Synonyms: PCNA; Proliferating cell nuclear antigen and 4 more synonyms used to describe this biomarker that Amplion uses in its algorithms. Associated Biomarker Indications. Gene symbol: PCNA: Gene name: proliferating cell nuclear antigen: Chromosome: 20: Chromosomal band: pter-p12: Imprinted: Unknown: Genomic reference: NC_000020.10: Transcript reference: NM_002592.2: Associated with diseases: ataxia-telangiectasia-like disorder 2: Citation reference(s)-Curators (1) Global Variome, with Curator vacancy: Total.
Gene Effect: Outcome from DEMETER2 or CERES. A lower score means that a gene is more likely to be dependent in a given cell line. A score of 0 is equivalent to a gene that is not essential whereas a score of -1 corresponds to the median of all common essential genes A mutation in the gene encoding pol η has been shown to be associated with the variant form of the genetic disease Xeroderma Pigmentosum(XP-V) (Masutani et al.,1999). Pol η binds to PCNA(Haracska et al., 2001b), as does the product of another disease-linked gene, the RecQ-like DNA helicase WRN the PCNA expression in the oocyte is unknown and needs to be elucidated. It has been demonstrated that PCNA is in-volved in DNA repair (Celis and Madsen, 1986; Toschi and Bravo, 1988; Shivji et al., 1992; Wood and Shivji, 1997), which suggests that PCNA may be expressed by cells that are not proliferating. Correspondingly, it has been hypoth Gene name (HAGRID: 113) HGNC symbol PCNA Aliases Common name proliferating cell nuclear antigen Potential relevance to the human ageing process Main reason for selection Entry selected based on evidence linking the gene product to a pathway or mechanism linked to ageing Description
Abstract. We have cloned the promoter of the human PCNA gene that codes for a proliferating cell-nuclear antigen. Two promoter DNA fragments were cloned of 1416 and 389 bp in length. Expression of luc and PDX1 genes under the control of the PCNA promoter was demonstrated in transient and stable transfection in human and mouse cell lines. We have shown that PCNA promoter within genetically. The anaphase-promoting complex/cyclosome (APC/C) is a cell cycle-regulated E3 ubiquitin ligase that controls the degradation of substrate proteins at mitotic exit and throughout the G1 phase. We have identified an APC/C substrate and cell cycle-regulated protein, KIAA0101/PAF15. PAF15 protein levels peak in the G2/M phase of the cell cycle and drop rapidly at mitotic exit in an APC/C- and KEN.
The proliferating cell nuclear antigen (PCNA) is a key protein in the eukaryotic DNA replication and cell proliferation. Following the cloning and characterisation of the human PCNA gene, the question of the existence of pseudogenes in the human genome was raised. In this short communication we summarise the existing information about the PCNA pseudogenes and critically assess their status Pcna Gene Detail Summary Symbol. Pcna Name. proliferating cell nuclear antigen. Feature Type. protein coding gene. IDs. MGI:97503 NCBI Gene: 18538. Alliance. gene page. Transcription Start Sites. 3 TSS. Location & Maps more. Sequence Map Chr2:132249286. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Shibahara, K., Stillman, B. Cell (1999) Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation. Xiong, Y., Zhang, H., Beach, D. Genes Dev. (1993 PCNA gene has been reported as a single copy per haploid genome in some species and as multiple copies in others. A single band was observed on Southern blots for the HindIII-digested genomi PrimePCR™ PreAmp for SYBR® Green Assay: Pcna, Rat Reaction: 400 reactions Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis
PCNA-associated factor, the protein encoded by the KIAA0101/PCLAF gene, is a cell-cycle regulated oncoprotein that regulates DNA synthesis, maintenance of DNA methylation, and DNA-damage bypass, through the interaction with the human sliding clamp PCNA. KIAA0101/PCLAF is overexpressed in various cancers, including hepatocellular carcinoma (HCC) The PCNA gene is one of the few inducible repair genes following radiation exposure, and it executes cellular responses to stress-repair or apoptosis. Overexpression of PCNA is used as a useful aid for investigation of proliferating cells (4) Sequence variants and/or copy number variants (deletions/duplications) within the PCNA gene will be detected with >99% sensitivity. Variants classified as unknown significance (VUS), likely pathogenic, or pathogenic will be reported. Benign and likel..
p21 Cip1 (alternatively p21 Waf1), also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a cyclin-dependent kinase inhibitor (CKI) that is capable of inhibiting all cyclin/CDK complexes, though is primarily associated with inhibition of CDK2. p21 represents a major target of p53 activity and thus is associated with linking DNA damage to cell cycle arrest The following PCNA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCNA cDNA ORF which is encoded by the open reading frame (ORF) sequence Numerous PCNA-associated proteins compete for binding to a common surface on PCNA; J. C. Mechanism of CRL4 Cdt2, a PCNA-dependent E3 ubiquitin ligase. Genes Dev. 25, 1568-1582 (2011) PCNA gene expression was significantly increased in siRNA-treated AB-MSCs after treatment with UC-EVs (P < 0.05) (fig. S3I), and protein abundance was also successfully restored in PCNA-knockdown AB-MSCs (fig. S3, G and J)
PCNA cloned gene : ORF from ATG to Stop, in pUNO1 expression plasmid selectable in E.coli and mammalian cells. Fully sequenced PrimePCR™ PreAmp for SYBR® Green Assay: Pcna, Mouse Reaction: 400 reactions Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis Functional Associations. PCNA-AS1 has 1,362 functional associations with biological entities spanning 3 categories (molecular profile, cell line, cell type or tissue, gene, protein or microRNA) extracted from 13 datasets
The PCNA labeling index (PCNA-Li) is a useful marker for predicting recurrence time and the patients' prognosis of HCC . However, the precise regulation of PCNA was still obscure. Here we identified an aslncRNA, named as PCNA-AS1 and located on the opposite strand of PCNA gene, was significantly up-regulated in HCC PCNA antibody detects PCNA protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX124496) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody We introduced the D150E mutation into the fission yeast PCNA gene (pcn1) together with a genetically linked selectable marker (natMX4). The mutant strain was viable and exhibited similar growth to the wild-type parental strain, and to a pcn1 + strain with the same natMX4 linked marker (data not shown) Anti-PCNA Antibody (PC10) is a mouse monoclonal IgG 2a κ PCNA antibody, cited in 2,539 publications, provided at 200 µg/ml; raised against rat PCNA made in the protein A expression vector pR1T2T; Anti-PCNA Antibody (PC10) is recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FC Gene symbol: PCNA: Gene ID (NCBI) 5111 Conjugate: Unconjugated: Form: Liquid: Purification Method: Protein A purification: Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage
The gene PCNA may have Genomic and Proteomic products available from Sigma-Aldrich Santa Cruz Biotechnology, Inc. offers a broad range of gene silencers in the form of siRNAs, shRNA Plasmids and shRNA Lentiviral Particles. PCNA gene silencers are available as PCNA siRNA, PCNA shRNA Plasmid, PCNA shRNA Lentiviral Particles. Gene silencers are useful for gene studies in combination with antibodies used for protein detection PCNA proliferating cell nuclear antigen. Overview; Perturbation Effects; Characterization; Description; Loading.. Complete information for PCNA-AS1 gene (RNA gene), PCNA antisense RNA 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendiu
Plasmid pCNA from Dr. Hongqing Fang's lab contains the insert I-CreI lamba Red and is published in PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. This plasmid is available through Addgene The putative gene for P. furiosus PCNA was excised by digestion with EcoRI and XhoI. It was then fused by ligation to the gene coding for maltose-binding protein in the vector pMAL (New England Biolabs) at the EcoRI and SalI sites. This construct was named pMPCNA. To insert the structural gene for the protein PfuPCNA into a pET21a (Novagen),
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. It achieves this processivity by encircling the DNA, thus creating a topological link to the genome. It is an example of a DNA clamp PCNA is a member of the so called DNA sliding clamp family, which also includes the E.coli DNA polymerase (pol) III β-subunit and the phage T4 gene45 protein (Kelman and O'Donnell, 1995; Wyman and Botchan, 1995) PCNA is a 36 KDa protein which is well-conserved in all eukaryotic species from yeast to human. The expression of PCNA has been reported in fetal and adult ovaries in several arthropods and mammals, but with considerable variation , , , , , , ,  Previously, using proteome analysis, we identified Proliferating cell nuclear antigen (Pcna) as one of the putative target genes of Hoxc8. Here, we asked whether Hoxc8 regulates Pcna expression by directly binding to the regulatory sequence of Pcna Proliferatingcellnuclearantigen(PCNA):ringmasterofthe genome T.PAUNESKU,S.MITTAL,M.PROTIC´,J.ORYHON,S.V.KOROLEV, A.JOACHIMIAKandG.E.WOLOSCHAK* (Received10November2000;accepted1June2001) Abstract. phylogenetictree(Almendraletal.1987,Kelman Proliferatingcellnuclearantigen(PCNA)proteinisoneofthe 1997,ShibaharaandStillman1999).PCNAhasbee
The protein called proliferating cell nuclear antigen (PCNA) was initially identified as an accessory factor for mammalian DNA polymerases—δ and ε and acted as an essential component of chromosomal DNA replisome (Bravo et al. 1987; Prelich et al. 1987; Choe and Moldovan 2017 ) genes in panel. prev next alkbh2 0 alkbh3 0 apex1 0 apex2 0 aplf 0 aptx 0 atm 0 atr 0 atrip 0 blm 0 brca1 0 brca2 0 brip1 0 ccnh 0 cdk7 0 cetn2 0 chaf1a 0 chek1 0 chek2 0 clk2 0 dclre1a 0 dclre1b 0 dclre1c 0 ddb1 0 ddb2 0 dmc1 0 dut 0 eme1 0 eme2 0 endov 0 ercc1 0 ercc2 0 ercc3 0 ercc4 0 ercc5 0 ercc6 0 ercc8 0 exo1 0 faap20 1 faap24 1 fan1 0 fanca 0 fancb 0 fancc 0 fancd2 0 fance 0 fancf 0. Removal of the proliferation component of gene expression by proliferating cell nuclear antigen (PCNA) adjustment via statistical methods has been addressed in numerous survival prediction studies for breast cancer and all cancers in the Cancer Genome Atlas (TCGA). These studies indicate that the removal of proliferation in gene expression by PCNA. Mutations of the PCNA domain of the CDKN1C gene are known to be associated with IMAGe syndrome thus with adrenal disease, although neither affected patient in our case had evidence of adrenal dysfunction. This report supports the previously reported findings of Russell-Silver syndrome. Welcome To The New PCNA.com To access new features, you'll need to reset your password the first time you sign in. Get Started
Proliferating cell nuclear antigen (PCNA) is an essential component of the DNA replication and repair machinery in the domain Eucarya. We cloned the gene encoding a PCNA homolog (PfuPCNA) from an euryarchaeote, Pyrococcus furiosus, expressed it in Escherichia coli, and characterized the biochemical proper-ties of the gene product Proliferativni ćelijski nuklearni antigen. Proliferativni ćelijski nuklearni antigen (PCNA) je DNK stega koja deluje kao faktor procesivnosti za DNK polimerazu δ u eukariotskim ćelijama i koja je esencijalna za replikaciju. PCNA je homotrimer koji ostvaruje svoju procesivnost putem obuhvatanja DNK, pri čemu ona deluje kao osnova na kojoj se vezuju. Search results for pcna at Sigma-Aldrich. Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta To investigate possible correlations between four genes, the protein interaction network (Figure 5(b)) of GTSE1, MCM6, CDC20, and PCNA was built with STRING. The PPI enrichment value of the network is 0.00304, which means the four proteins are at least partially biologically connected as one group The prokaryotic sliding clamp is a protein referred to as β and the eukaryotic sliding clamp is called proliferating cell nuclear antigen (PCNA). The T4 bacteriophage also utilizes a ring-type polymerase; its sliding clamp, called gene protein 45, is a trimer similar to PCNA (see Figure 1), but lacks homology to either PCNA or β [1,2]
between N-myc gene amplification and proliferation activity. Proliferating cell nuclear antigen (PCNA) is a proliferation-induced 36 kD nuclear protein that is the auxiliary component of DNA polymerase b PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene Proliferating cell nuclear antigen (PCNA), a eukaryotic DNA replication factor, functions not only as a processivity factor for DNA polymerase delta but also as a binding partner for multiple other factors Gene/Insert PI ; 14822: pRD119 (PCNA GFP) PCNA-GFP (Drosophila melanogaster) Duronio Add to Cart 26461: pEGFP-PCNA-IRES-puro2b: PCNA (Homo sapiens) Gerlich Add to Cart 54221: mEmerald-PCNA-19: PCNA-19 (Homo sapiens) Davidson Add to Cart 54222: mEmerald-PCNA-19-NLS-4: PCNA-19 NLS (Homo sapiens) Davidso
The following Pcna gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcna cDNA ORF which is encoded by the open reading frame (ORF) sequence PCNA (proliferating cell nuclear antigen) is a protein related to tumor development, which has been used extensively in breast cancer diagnosis and prognosis. PCNA has proven to be a useful marker to evaluate cell proliferation and prognosis when combined with other breast cancer markers. Construction of PCNA promoter luciferase reporter plasmid will provide the theory basis for researching.
The gene coding for proliferating cell nuclear antigen (PCNA) was identified in Dunaliella tertiolecta (Chlorophyceae) and Isochrysis galbana (Prymnesiophyceae). Southern blot hybridization using a PstI fragment of rat PCNA gene (pCR-1) as a probe showed that there is apparently a single copy of this gene per haploid genome in both species The Proliferating Cell Nuclear Antigen (PCNA) gene is a growth-regulated gene, whose expression is under the control of both transcriptional and posttranscriptional mechanisms. In previous work, it was shown that the 73 bp immediately upstream of the CAP site and intron 4 are major regulatory elements
wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound pcna - proliferating cell nuclear antigen. Danio rerio. Synonyms: PCNA,. PCNA antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 10205-2-AP. KD/KO Validated The cyclin-dependent kinase inhibitor p21waf1/cip1 is known to impair DNA synthesis by binding to PCNA, the co-factor of DNA polymerases δ and ε PCNA 25 Feature Type ORF Click Gene Ontology Details to view all GO information and evidence for this locus as well as biological processes it shares with other genes. Summary Sliding clamp for DNA polymerase delta; involved in DNA. Phenotype data for mouse gene Pcna. Discover Pcna's significant phenotypes, expression, images, histopathology and more. Data for gene Pcna is all freely available for download
Lysine residues of non-histone proteins including proliferating cell nuclear antigen (PCNA) and p53 are also monomethylated. As a consequence, the methyltransferase activity of the enzyme is implicated in many essential cellular processes including DNA replication, DNA damage response, transcription modulation, and cell cycle regulation PCNA gene (Ottavio et al. 19906). None of the PCNA constructs mentioned above nor the endogenous PCNA gene are expressed in Gi-specific ts mutants of the cell cycle stimulated at the restrictive temperature (Jaskulski et al. 19886), a feature that the PCNA gene shares with other genes coding for the proteins of DNA synthesizing machinery, and. Product Data: Gene ID: 5111: Forward Sequence: CAAGTAATGTCGATAAAGAGGAGG: Reverse Sequence: GTGTCACCGTTGAAGAGAGTGG: Accession No: NM_002592, NM_182649, BC000491.